Arrange the various steps of DNA fingerprinting technique in the correct order
1) Separation of DNA fragments by electrophoresis
2) Digestion of DNA by restriction endonucleases
3) Hybridization using labelled VNTR probe
4) Isolation of DNA
5) Detection of hybrised DNA fragments by autoradiography
6) Transferring the separated DNA fragments to nitrocellulose membrane
4 --> 2 --> 1 --> 6 --> 3--> 5
4 --> 1 --> 2 --> 3 --> 6--> 5
2 --> 1 --> 4 --> 6 --> 3--> 5
3 --> 5 --> 4 --> 2 --> 1 --> 6
As we have learned
DNA Finger Printing -
This is a quick way to compare the DNA sequence of any two DNA samples. This technique is based on VNTR, which are specific to every individuals
- wherein
VNTR are intronic reason this technique was developed by Alec Jeffreys