Barley endosperm bioassay
- Endosperms are detached from embryos, sterilized and allowed to remain in 1 ml of test solution for 1-2 days.
- There is a build-up of reducing sugars.
- The content of reducing sugar is proportional to gibberellin concentration.
- Reducing sugars are not formed in a control experiment where endosperms are kept in plain water.
- Hence, barley endosperm bioassay is to test the activity of gibberellin
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