During denaturation in the process of PCR , DNA is heated to
120 -125 oC
94-95 oC
40-45 oC
55-60 oC
As we have learned
Denaturation -
This alsp known as melting of target DNA. The target DNA is heated to , resulting breaking down of H-bonds and separation of two strands
- wherein
Each strand acts as template for DNA synthesis in direction