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  • The correct sequence of making a cell competent is Option: 1 treatment with divalent cations --> incubation of cells with recombinant DNA on

The correct sequence of making a cell competent is 

Option: 1

treatment with divalent cations --> incubation of cells with recombinant DNA on ice --> heat shock (42oC)--> placing on ice 


Option: 2

heat shock (42oC)--> incubation of cells with recombinant DNA on ice --> treatement with divalent cations --> placing on ice 


Option: 3

treatment with divalent cations --> placing on ice --> incubation of cells with recombinant DNA on ice --> heat shock (42oC) 


Option: 4

incubation of cells with recombinant DNA on ice --> heat shock (42oC)--> treatment with divalent cations --> placing on ice 


Answers (1)

As we have learned

Heat Shock Method -

This technique is usually applied in transformation of recombinant DNA in bacteria. 42°C for 90 seconds makes bacterial cell membrane porous, allowing DNA to be introduced into the cells.

- wherein

CaCl2 is used, which generates membrane potential surrounding the plasma membrane.

 

 

 

Posted by

Ramraj Saini

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