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Get Answers to all your Questions
- #National Eligilibility Cum Entrance Test
- #Class 11
- #Cell Structure and Function
- #Medical
- #Biology
- #National Eligibility Cum Entrance Test
Which of the following is present in the maximum quantity in the cellular mass?
Option: 1
Proteins
Option: 2
Amino acids
Option: 3
Water
Option: 4
All
Answers (1)
Analysis of Chemical Composition in Living Tissues
It can be done by the following two methods:
Chemical Analysis:
- By grinding any living tissue (like a piece of vegetable) with trichloroacetic acid (CCl3COOH) using a mortar and pestle.
- A thick slurry obtained is strained with a cheesecloth.
- Two fractions would be obtained as:
- Filtrate or acid-soluble pool
- Retentate or acid insoluble fraction
- The acid-soluble pool consisted of macromolecules.
- Their molecular weight is less than 1000 Da.
- Amino acids, sugars, and nucleotides are macromolecules.
- The acid-insoluble pool consisted of macromolecules whose molecular weight is more than 1000 Da.
- Carbohydrates, nucleic acids, and proteins are macromolecules.
- The acid insoluble fraction also included lipids. However, lipids are micromolecules as they never weigh more than 800 Da. This happened because the lipid components of the plasma membrane and organelle membranes form vesicles which are insoluble. So, they appear along with acid insoluble fraction despite being a micromolecule.
Hence, the correct Option c.
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