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Which of the following equipments is essentially required for growing microbes on a large scale, for industrial production of enzymes?

 

  • Option 1)

    BOD incubator

  • Option 2)

    sludge digestor 

  • Option 3)

    Industrial oven

  • Option 4)

    Bioreactor

  Bioreactors - Bioreactors are stirrer cylindrical tanks. The stirrer provides movement, even mixing and O2 availability throughout the bioreaction. - wherein Bioreactors can be 100 to 1000 lt for large production.       In order to produce large quantities of the enzymes , bioreactors are used wherein large volumers of culture can be processed or provides optimal conditions for achieving...

Following statements describe the characteristics of the enzyme Restriction Endonuclease. Identify the incorrect statement.

  • Option 1)

    The enzyme cuts DNA molecule at identified position within the DNA.

  • Option 2)

    The enzyme binds DNA at specific sites and cuts only one of the two strands.

  • Option 3)

    The enzyme cuts the sugar-phosphate backbone at specific sites on each strand.

  • Option 4)

    The enzyme recognises a specific palindronic nucleotide sequence in the DNA.

  Restriction Endonuclease - These enzymes cleave DNA only within or near the specific base sequence. These sequences are called as recognition sites. They are of three types RE- I, RE-II and RE-III. - wherein 1st Restriction enzyme (Hind II) was used in RDT.      Restriction endonuclease bind to the specific sites (palindromic sequences) the  and cut both the strands of the .Option 1)The...

What triggers activation of protoxins to active Bt toxin of Bacillus thuringinensis in boll worm?

  • Option 1)

    Body temperature

  • Option 2)

    Moist surface of midnight

  • Option 3)

    Alkaline pH of gut

  • Option 4)

    Acidic pH of stomach

  Bacillus Thuringiensis - Bacillus Thuringiensis is a Gram-positive bacteria; During sporulation this bacteria  forms crystals of proteins or (cry protein), which are coded by cry genes (Cry 1 AC) Cry genes are used to make insecticide transgenic plants. - wherein When insects ingest crystal proteins , the alkaline pH of gut denature the insoluble protein into soluble. Soluble proteins makes...

Which of the following is true for Golden rice?

 

  • Option 1)

    It is a Vitamin A enriched, with a gene from daffodil.

     

  • Option 2)

    It is pest resistant, with gene from Bacillus thuringiensis

  • Option 3)

    It is drought tolerant, developed using Agrobacterium vector

  • Option 4)

    It has a yellow grains, because of a gene introduced from a primitive variety of rice.

     

Option 1)It is a Vitamin A enriched, with a gene from daffodil.  Option 2)It is pest resistant, with gene from Bacillus thuringiensisOption 3)It is drought tolerant, developed using Agrobacterium vectorOption 4)It has a yellow grains, because of a gene introduced from a primitive variety of rice.  
Medical
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Which of the following restriction enzymes produces blunt ends?

  • Option 1)

    Sal I

  • Option 2)

    Eco RV

  • Option 3)

    Xho I

  • Option 4)

    Hind III

 
None As discussed in Palindromic sequence for AluI - - wherein Produce blunt ends.    Blunt ends have no overlap. Restriction enzymes cut DNA at same position EcoRV produces blunt  ends Option 1) Sal I This is incorrect option Option 2) Eco RV This is correct option Option 3) Xho I This is incorrect option Option 4) Hind III This is incorrect option
Medical
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Which of the following is not a component of downstream processing?

  • Option 1)

    Separation

  • Option 2)

    Purification

  • Option 3)

    Preservation

  • Option 4)

    Expression

 
  Downstream Processing - Separation and purification of desired product before marketing. -    Downstream Processing doesnot require expression of proteins. Option 1) Separation Incorrect Option 2) Purification Incorrect Option 3) Preservation Incorrect Option 4) Expression Correct
Medical
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Which kind of therapy was given in 1990 to a four-year-old girl with adenosine deaminase (ADA) deficiency?

  • Option 1)

    Gene therapy

  • Option 2)

    Chemotherapy

  • Option 3)

    Immunotherapy

  • Option 4)

    Radiation therapy    

 
  Gene Therapy in Human - 1st successfull gene transfer in human was performed by F. Anderson. Ashi Disilva a 4 year girl was treated by replacing ADA gene (Adenosine deaminase deficiency in lymphocytes. - wherein Girl was suffering from SCID (Severe combined Immuno deficiency).    Gene Therapy was used for treatment of ADA deficiency. Option 1) Gene therapy Correct Option...
Medical
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Stirred-tank bioreactors have been designed for

  • Option 1)

    purification of product

  • Option 2)

    addition of preservatives to the product

  • Option 3)

    availability of oxygen throughout the process

  • Option 4)

    ensuring anaerobic conditions in the culture vessel

 
As discussed in Bioreactors - Bioreactors are stirrer cylindrical tanks. The stirrer provides movement, even mixing and O2 availability throughout the bioreaction. - wherein Bioreactors can be 100 to 1000 lt for large production.    Bioreactors are used for mixing and  availability throughout the bio reaction. Option 1) purification of product Incorrect Option 2) addition of preservatives to...
Medical
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A foreign DNA and plasmid cut by the same restriction endonuclease can be joined to form a recombinant plasmid using

  • Option 1)

    Eco R1

  • Option 2)

    Taq Polymerase

  • Option 3)

    Polymerase III

  • Option 4)

    ligase

 
As learnt in Ligase - These enzymes form phosphodiester bonds between adjacent nucleotides and covalently link two fragments of double-stranded DNA. -    Ligase are enzymes that join two phosphodiester bonds thus, forming recombinant plasmid. Option 1) Eco R1 This answer is incorrect Option 2) Taq Polymerase This answer is incorrect Option 3) Polymerase III This answer is incorrect Option...
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