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  • A corn farmer has perennial problem of corn - borer infestation in his crop. Being environmentally conscious he does not want to spray insecticides. Suggest solution based on your knowledge of biotechnology. Write the steps carried out to achieve it.

A corn farmer has perennial problem of corn - borer infestation in his crop. Being environmentally conscious he does not want to spray insecticides. Suggest solution based on your knowledge of biotechnology. Write the steps carried out to achieve it.

 

 

 

 
 
 
 
 

Answers (1)

(i) Bt toxin gene should be extracted from Bacillus thuringeinesis 

(ii) Bt toxin gene is then closed from bacteria and expressed in corn to provide resistance to corn borer without the need of insecticide.

(iii) The toxin is coded by a gene named cry. The protein encoded by gene cryIAb controls corn borer without causing harm to the environment.

(iv) Bt toxin protein exists as inactive protoxins but once an insect ingests the inactive toxin, it is converted into the active form of toxin due to alkaline pH of the gut which solubilizes the crystals which contain toxic insecticidal protein. The activated toxins bind the surface of midgut epithelial cells and create pores that cause cell swelling and lysis and eventually cause the death of insects (corn borer).

Posted by

Priyanka Kumari

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