(a) Describe the process of transcription in bacteria.
(b) Explain the processing the hRNA needs to undergo before becoming functional mRNA in eukaryotes.
(a) Initiation - Enzyme (DNA dependent RNA polymerase) RNA polymerase binds with sigma factor and attaches to the promoter site , i.e, 5' site of the DNA.
Elongation - When RNA polymerase moves from the promoter to the terminator site it causes the polymerisation of nucleoside triphosphate / nucleotides resulting in the formation of RNA (in the 5' - 3' direction )
Termination - RNA polymerase on reaching the terminator site binds to p factor and the (nascent transcribesd) RNA falls off along with RNA polymerase.
(b) hRNA undergoes splicing where introns are removed and exons are joined in a defined order.
hnRNA undergoes additional processing, i.e, capping (addition to the 5' end), tailing (200 - 300 polyadenylated residue are added to the 3' end).