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  • (a) Write the mechanism that enbales Agrobacterium tumefaciens to develop tumors in their host dicot plant. (b) State how Agrobacterium tumefaciens and some retroviruses have been modified as useful cloning vectors.

(a) Write the mechanism that enbales Agrobacterium tumefaciens to develop tumors in their host dicot plant.

(b) State how Agrobacterium tumefaciens and some retroviruses have been modified as useful cloning vectors.

 

 

 

 
 
 
 
 

Answers (1)

a) Agrobacterium tumifaciens, a pathogen of several dicot plants is able to deliver a piece of DNA known as ‘T-DNA’ to transform normal plant cells into a tumor and direct these tumor cells to produce the chemicals required by the pathogen.

b) The tumor inducing (Ti) plasmid of Agrobacterium tumifaciens has now been modified into a cloning vector which is no more pathogenic to the plants but is still able to use the mechanisms to deliver genes of our interest into a variety of plants. Similarly, retroviruses have also been disarmed and are now used to deliver desirable genes into animal cells. So, once a gene or a DNA fragment has been ligated into a suitable vector it is transferred into a bacterial, plant or animal host (where it multiplies).

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Priyanka Kumari

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