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List the steps of preparation of temporary mount of a leaf peel to observe stomata.

 

 

 

 
 
 
 
 

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The following steps are :

1. Remove a healthy leaf from the plant.

2. Remove a part of peel from the lower surface of the leaf. The peel can be removed by folding the leaf over and gently pulling the peel a part using forceps. place it in glass have water.

3. Put a few drops of safranin stain on it.

4. After 2-3 min put the peel on a glass slide.

5. Put a drop of glycerin over peel and place a coverslip over it with the help of needle to avoid air bubbles.

6. Remove excess stain and glycerin with the help of blotting paper.

7. Observe slide under low and high power magnification of a compound microscope.

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Sumit Saini

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