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How will you distinguish and
hydroxyl groups present in glucose? Explain with reactions.
Answers (1)
To distinguish between the and
hydroxyl groups of glucose via the chemical method, presence of 5-OH groups can indicate. The reaction involves adding a few drops of a cone.
in the presence of pyridine, this results in the formation of forms Penta-acetyl derivative. This also indicates that from 5-OH groups, one group is primary (1°) alcoholic while others are secondary (2°) alcoholic groups.
The other way to determine their presence is by oxidising gluconic acid (Glucose) with HNO3, this forms a dicarboxylic acid named saccharic acid. The result indicates that only one of the -OH group oxidised and formed -COOH group and the rest of the secondary -OH groups are unable to oxidise due to absence of drastic conditions.
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