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The specific palindromic sequence which is recognized by EcoRI is :
 

Option: 1 \\5'- GAATTC-3'\\3'-CTTAAG-5'
 

Option: 2 \\5'- GGAACC-3'\\3'-CCTTGG-5'
 

Option: 3 \\5'- CTTAAG-3'\\3'-GAATTC-5'
 

Option: 4 \\5'- GGATCC-3'\\3'-CCTAGG-5'

The palindrome identified by EcoRI is 5’-GAATTC-3’ / 3’-CTTAAG-5’. Hence, the correct option is (a).

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Posted by

Ritika Jonwal

Bt cotton variety that was developed by the introduction of toxin gene of Bacillus thuringiensis (Bt) in resistant to :
Option: 1 Insect pests
Option: 2 Fungal diseases
Option: 3 Plant nematodes
Option: 4 Insect predators  

Bt toxins from Bacillus thuringiensis kills Bollworm that eats the cotton plant. 

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Ritika Jonwal

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Which of the following statements is not correct ?
Option: 1  In man insulin is synthesised as a proinsulin
Option: 2 The proinsulin has an extra peptide called C-peptide
Option: 3 The functional insulin has A and B chains linked together by hydrogen bonds
Option: 4 Genetically engineered insulin is produced in E-coli.  

The functional insulin has A and B chain bonded by the disulfide bridge. Hence, the correct option is (c).

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Posted by

Ritika Jonwal

The sequence that controls the copy number of the linked DNA in the vector, is termed:
 
Option: 1 Selectable marker
Option: 2 Ori-site
Option: 3 Palindromic sequence  
Option: 4  Reognitions site

Ori-site is the site where DNA replication begins and controls the copy number of the linked DNA.

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Posted by

Ritika Jonwal

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Match the following columns and select the correct option.   

     Column -I                                           Column -II

 (a) Bt-cotton                                         (i) Gene therapy

(b) Adenosine deaminase deficiency   (ii) Cellular defence

(c) RNAi                                               (iii) Detection of HIV infection

(d) PCR                                               (iv) Bacillus thuringiensis
Option: 1 (a)         (b)        (c)         (d)  
                  iv)         (i)       (ii)          (iii)
Option: 2 (a)         (b)        (c)         (d)  
                 (iii)         (ii)        (i)          (iv)
Option: 3 (a)         (b)        (c)         (d)  
                  (ii)         (iii)        (iv)         (i)
Option: 4 (a)         (b)        (c)         (d)  
                  (i)         (ii)       (iii)         (iv)

(a)         (b)        (c)         (d)  
iv)         (i)       (ii)          (iii)

 

 (a) Bt-cotton  -   Bacillus thuringiensis                            

(b) Adenosine deaminase deficiency  -  Gene therapy 

(c) RNAi -    Cellular defence                                      

(d) PCR - Detection of HIV                                               

 

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Posted by

Ritika Jonwal

In ............ separated DNA fragments can be visualized with the help of:
Option: 1 AAcetocarmine in bright blue light
 
Option: 2 Ethidium bromide in UV radiation
 
Option: 3 Acetocarmine in UV radiation
 
Option: 4 Ethidium bromide in infrared radiation
 

In AGE or Agarose Gel Electrophoresis, DNA fragments can be separated and visualized with the help of EtBr in UV radiation. Hence, the correct option is (b).

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Ritika Jonwal

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Why are certain animals called ‘transgenic’ ? Give an example of such an animal that is being used for testing the vaccine safety for a specific human disease. Name the disease.

 

 

 

 
 
 
 
 

The animal carrying the introduced foreign gene for improving their yield is said to be a transgenic animal.

Mice are being used for testing vaccine safety for a specific human disease like influenza viruses.

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Posted by

Priyanka Kumari

Name any two most common bio-reactors and mention their importance in biotechnology.

 

 

 

 
 
 
 
 

1. Simple Stirred-tank reactor

2. Sparged stirred-tank bioreactor

Importance of Bioreactors:  

100 - 1000 litres of culture can be processed at a time.
It provide optimal conditions for getting the desired product by providing optimum growth conditions like

  • temperature,
  • pH,
  • substrate,
  • salts,
  • vitamins and
  • oxygen.
     

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Priyanka Kumari

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Silencing of a gene could be achieved through the use of:

a. RNAi only
b. antisense RNA only
c. both RNAi and antisense RNA
d. none of the above

The answer is the option (c) both RNAi and antisense RNA

Explanation: (c) both RNAi and antisense RNA

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infoexpert25

ADA is an enzyme which is deficient in a genetic disorder SCID. What is the full form of ADA?

a. Adenosine deoxyaminase
b. Adenosine deaminase
c. Aspartate deaminase
d. Arginine deaminase

The answer is the option (b) Adenosine deaminase

Explanation: (b) Adenosine deaminase commonly called ADA.

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infoexpert25

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