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  • In the above represented plaamd an alien piece of DNA is inserted af Ecoli site.

Q.173) style="margin-right:8px"> In the above represented plaamd an alien piece of DNA is inserted af Ecoli site. Which of the following strategies vill be chosen to select the recombinant colonies!

 

A) style="margin-right:8px">Blue color colonies grown on ampicillin plates can be selected

 

B) style="margin-right:8px">Using ampicillin tetracycon containing medium plate

C) style="margin-right:8px">Blue color colongs will be selected.

 

D) style="margin-right:8px">White color collies will

 

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Q.173) style="margin-right:8px">In plasmids like pUC19, the lacZ gene is used for blue-white screening. When foreign DNA is inserted into the lacZ region, the gene becomes disrupted, preventing the production of β-galactosidase. As a result, colonies with recombinant DNA appear white because X-gal cannot be cleaved, while non-recombinant colonies, which still have an intact lacZ gene, turn blue. Therefore, to select recombinant colonies, white color colonies should be chosen. 

Hence, the correct answer is option 4.

 

 

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Shreya Pandey

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