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(a) Why must bacterial cells be first made ‘competent’ in r-DNA technology ? How is the process carried ?

(b) Name the method by which an alien DNA can be made to enter (i) plant cell; (ii) animal cell.

 

 

 

 
 
 
 
 

Answers (1)

(a)

Since DNA is hydrophilic, it cannot pass through cell membrane hence bacterial cell is made competent by treatment with a specific concentration of a divalent cation such as Ca++ which increase the efficiency of entry of DNA through the pores of the cell wall.

(b) 

(i) Plants cell- biolistic / gene germs

(ii) Animal cells - Microinjection

Posted by

Priyanka Kumari

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