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  • How does -galactosidase coding sequence act as a selectable marker ? Why is it a preferred selectable marker to antibiotic resistance genes ? Explain.  

How does \beta-galactosidase coding sequence act as a selectable marker ? Why is it a preferred selectable marker to antibiotic resistance genes ? Explain.

 

 

 

 
 
 
 
 

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Alternative selectable markers have been developed which differentiate recombinants from non-recombinants on the basis of their ability to produce colour in the presence of a chromogenic substrate. In this, a recombinant DNA is inserted within the coding sequence of an enzyme, β-galactosidase.

This results into inactivation of the enzyme, which is referred to as insertional inactivation. The presence of a chromogenic substrate gives blue coloured colonies if the plasmid in the bacteria does not have an insert. The presence of insert results into insertional inactivation of the β-galactosidase and the colonies do not produce any colour, these are identified as recombinant colonies.

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Priyanka Kumari

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