Mention the role of (i) selectable marker, (ii) Ori and (iii) rop in E. coli cloning vector pBR322.
(i) Selectable marker - Kelps in identifing and eliminating non transfformants and selectively permitting the growth of the transformants.
(ii) Ori - helps to start replication and any piece of DNA when linked to this sequence can be made to replicate within host cell respoinsible for controlling the copy number of the linked DNA.
(iii) Codes for the proteins involved in replication of plasmid.