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  • Among the following statements, identify the ones that contain incorrect information: (i) Restriction endonucleases are obtained only from prokaryotes. <

Among the following statements, identify the ones that contain incorrect information:

(i) Restriction endonucleases are obtained only from prokaryotes.

(ii) Sma I creates blunt ends in plasmids.

(iii) Blunt ends can be made sticky with the help of enzyme phosphatase.

(iv) The size of pBR322 is 43 kb.

(v) Shuttle vectors possess two types of ori-sites.

Choose the option(s) that contain incorrect statements:

Option: 1

(i) and (iii)


Option: 2

(ii) and (iv)


Option: 3

(ii) and (v)

 


Option: 4

(iii) and (iv)


Answers (1)

best_answer

Statement (ii) which states that  Sma I creates blunt ends in plasmids is incorrect. Sma I is a restriction endonuclease that recognizes and cleaves DNA at a specific sequence, but it creates cohesive or sticky ends (overhangs) rather than blunt ends.

Statement (iv) which states that the size of pBR322 is 43 kb is incorrect because the size of pBR322 is approximately 4.4 kilobases (kb), not 43 kb.Hence, the correct answer is option 2.

 

Posted by

Devendra Khairwa

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