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  • Confused! kindly explain, Which of the following is not required for any of the techniques of DNA fingerprinting available at present?

Which of the following is not required for any of the techniques of DNA fingerprinting available at present?

  • Option 1)

    Polymerase chain reaction

  • Option 2)

    Zinc finger analysis

  • Option 3)

    Restriction enzymes

  • Option 4)

    DNA-DNA hybridization

 

Answers (1)

As we discussed

Steps of DNA fingerprinting -

(a) Extraction of DNA from the sample cells.

(b) Amplification of DNA by PCR

(c) Restriction digestion of PCR Products

(d) Separation on Agarose gel electrophoresis

(e) Southern blotting

(f) Hybridization using labelled VNTR probe

(g) Detection by autoradiography

- wherein

This technique is widely used in forensic science for criminal cases

 

 In DNA fingerprinting PCR Restriction enzymes and hybridisation techniques are used.


Option 1)

Polymerase chain reaction

Incorrect

Option 2)

Zinc finger analysis

Correct

Option 3)

Restriction enzymes

Incorrect

Option 4)

DNA-DNA hybridization

Incorrect

Posted by

Vakul

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