DNA fragments generated by the restriction endonucleases in a chemical reaction can be separated by:
Restriction mapping
Centrifugation
Polymerase chain reaction
Electrophoresis
When restriction endonuclease treats DNA, it generates a variety of DNA fragments. By using gel electrophoresis, these fragments are separated. Using an electric field and a gel matrix, gel electrophoresis is a method for separating biomolecules.