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  • From a given fragment of DNA, the objective is to isolate only the gene 'C' without any flanking sequences, which of the following enzyme given below  would be the suitable choice?

From a given fragment of DNA, the objective is to isolate only the gene 'C' without any flanking sequences, which of the following enzyme given below  would be the suitable choice?

 

Option: 1

Eco Rl

 


Option: 2

Eco Rl and Cla I


Option: 3

Cla I


Option: 4

Bam HI


Answers (1)

best_answer
  • If the objective is to isolate only the gene 'C' without any flanking sequences from a given DNA fragment, the suitable choice of enzyme would be Bam HI.
  • Bam HI is a restriction enzyme that recognizes and cuts DNA at specific palindromic sequences. By using Bam HI, the DNA fragment can be cleaved, separating the gene 'C' from the flanking sequences.
  • Unlike Eco Rl and Cla I mentioned in the other options, which may have additional recognition sites within the gene 'C' or the flanking sequences, Bam HI specifically targets and cuts at a sequence that is present only in the desired gene 'C'. This allows for the isolation of the gene 'C' without any contamination from the flanking sequences.
  • Therefore, the correct answer is option (4) - Bam HI.
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