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  • Given that, Plasmid-A yielded two fragments and Plasmid-B yielded three fragments after digestion with BamHI and analyzed by agarose gel electrophoresis.By observing we can make the following infer

Given that, Plasmid-A yielded two fragments and Plasmid-B yielded three fragments after digestion with BamHI and analyzed by agarose gel electrophoresis.By observing we can make the following inferences that 

1. Plasmid-A has two restriction sites and is circular.

2. Plasmid-B has three restriction sites and is circular.

3. Plasmid-A has two restriction sites and is circular.

4. Plasmid-B has two restriction sites and is linear.

On the basis of inferences choose the correct statement

 

Option: 1

1 and 2

 


Option: 2

2 and 3


Option: 3

1 and 4 

 


Option: 4

3 and 4


Answers (1)

best_answer
  • Based on the given information, Plasmid-A produced two fragments and Plasmid-B produced three fragments after digestion with BamHI and analyzed by agarose gel electrophoresis.
  • Inference 3 suggests that Plasmid-A has two restriction sites and is circular. This inference is supported by the observation of two fragments obtained from Plasmid-A digestion. The presence of two restriction sites indicates that the BamHI enzyme cleaves the plasmid at two specific locations, resulting in two fragments.
  • Inference 4 states that Plasmid-B has two restriction sites and is linear. This inference is based on the observation of three fragments obtained from Plasmid-B digestion. The presence of two restriction sites suggests that the BamHI enzyme cleaves the plasmid at two specific locations, resulting in three fragments due to the linear nature of Plasmid-B.
  • Therefore, the correct statement is option 4, as it includes 
    • Plasmid-A has two restriction sites and is circular.
    • Plasmid-B has two restriction sites and is linear.
  • Hence, the correct answer is option 4.
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manish

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