The 3'-5' phosphodiester linkages inside a polynucleotide chain serve to join
One DNA strand with the other DNA strand
One nucleoside with another nucleoside
One nucleotide with another nucleotide
One nitrogenous base with pentose sugar
Each strand of DNA is made up of several nucleotides. Contiguous nucleotides of the same strand are linked by 3'-5' phosphodiester bonds, ie. 3' carbon from one sugar and 5' carbon from another sugar. This bond is a strong covalent bond formed by a phosphate group and the ester groups on two pentose sugars. Hence option 2 is correct.