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The colonies of recombinant bacteria appear white in contrast to blue colonies of non-recombinant bacteria because of:
Inactivation of glycosidase enzyme in recombinant bacteria
Non-recombinant bacteria containing beta-galactosidase
Insertional inactivation of alpha-galactosidase in non-recombinant bacteria
Insertional inactivation of alpha-galactosidase in recombinant bacteria
Answers (1)
Blue-white screening allows detecting rapidly the recombinant bacteria in vector-based cloning experiments. The presence of an active \betaβ-galactosidase can be detected by X-gal, a colourless analogue of lactose, that may be cleaved to form a bright blue insoluble pigment. This results in a characteristic blue colour in cells containing the functional \betaβ-galactosidase. Blue colonies, therefore, show that they may contain a vector, while white colonies indicate the presence of an insert in lacZ \alphaα which disrupts the formation of an active \betaβ-galactosidase.
Hence, the correct answer is 'Insertional inactivation of alpha-galactosidase in recombinant bacteria'
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