What is the palindromic sequence of HindIII Restriction Enzymes?
5'- GAATTC - 3'
3' - CTTAAG - 5'
5' - AAGCTT - 3'
3'- TTCGAA - 5'
5' - GGATCC - 3'
3' - CCTAGG - 5'
None of above
5’GAATTC3′
3’CTTAAG5′
This is the palindromic sequence for EcoRI Restriction Enzymes isolated from E.coli.
B) HindIII is a type II restriction endonuclease that is isolate from isolated from the Haemophilus influenzae cleaving DNA palindromic sequences. The cofactor involved is Mg2+ through the process of hydrolysis. The palindromic sequence of HindIII Restriction Enzymes is
5’AAGCTT3′
3’TTCGAA5′.