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8. What are the conditions necessary for fixation of atmospheric nitrogen by Rhizobium? What is their role in
Answers (1)
The conditions necessary for fixation of atmospheric nitrogen by Rhizobium and their roles in nitrogen fixation are as follows:
1. Reducing environment- A reducing environment is necessary for the action of enzyme nitrogenase which is sensitive to free oxygen.
2. Presence of nitrogenase enzyme- a Mo-Fe protein — that helps in the conversion of atmospheric free nitrogen into ammonia.
3. Presence of leghaemoglobin- The root nodules containing Rhizobium contains leg-haemoglobin, which protects nitrogenase from oxygen.
4. Source of energy as ATP- Nitrogen fixation is energy requiring process thus ATP is required.
5. Source of reducing power NAD(P), H2 or FMNH2-
6. Ferredoxin as the electron donor- Ferredoxin makes nitrogen reactive.
7. Magnesium ions as cofactors- Magnesium act as a cofactor and helps in the activity of nitrogenase.
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