Which one is a true statement regarding DNA polymerase used in PCR

  • Option 1)

    It is used to ligate introduced DNA in recipient cell

  • Option 2)

    It serves as a selectable marker

  • Option 3)

    It is isolated from a virus

  • Option 4)

    It remains active at high temperature

 

Answers (1)

 

Working of RNA polymerase -

The RNA polymerase binds it promoter and initiates transcription ( Initialion) . It uses nucleoside triphosphate as substrate and polymereses in a template dependent fashion following the rule of complementarity.

- wherein

It somehow also facilities the opening of the helix and continues elongation.

 

Polymerase chain reaction (PCR) is used to amplify a DNA segment to synthesize invitro the multiple copies of gene (or DNA) of interest, using two set of primer and the enzyme DNA polymerase. This enzyme is isolated from a bacterium thermus aquatics and it remain active during high temperature.

 


Option 1)

It is used to ligate introduced DNA in recipient cell

Incorrect

Option 2)

It serves as a selectable marker

Incorrect

Option 3)

It is isolated from a virus

Incorrect

Option 4)

It remains active at high temperature

Correct

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