Which of the following can we use to confirm the presence of OH groups ?
Fehlings solution
Tollen's reagent
Acetic anhydride
Hydrogen cyanide
As we have learnt,
The number of hydroxy groups in a carbohydrate can be detected by acetylation reaction with acetyl chloride or acetic anhydride.
Acetylation leads to an increase in the mass of the compound and knowing the exact increase in mass, the number of hydroxyl groups can be determined.
Glucose reacts with acetic anhydride and forms glucose pentacetate
Hence, the correct answer is Option (3)
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