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  • Differentiate between the two newly synthesised strands within the fork

a. Differentiate between the two newly synthesised strands within the fork.

b. Name the enzyme involved in the process of DNA replication.

c. Name the eukaryote where the semiconservative mode of replication was experimentally proved.

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a. Difference between the two newly synthesised strands within the fork- Leading and lagging strand is given below:

Feature Leading strand Lagging strand
Synthesis direction Synthesized continuously in 5' → 3' direction Synthesized discontinuously in short fragments (Okazaki fragments)
Template orientation 3' → 5' towards the replication fork 5' → 3' away from the replication fork
Type of synthesis Continuous Discontinuous

b. Enzyme name- DNA polymerase. It adds nucleotides to the growing DNA strand using the parent strand as a template.

c. Example- Vicia faba (a type of broad bean). The semiconservative replication in eukaryotes was proved by Taylor, Woods, and Hughes using Vicia faba root tips with autoradiography.

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Saniya Khatri

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