The karyokinesis of mitosis can be studied under the following heads:
1. Prophase:
- During this stage, the chromatin condenses and the chromosomes are visible.
- The nucleolus disappears and the nuclear envelope fragments.
- With the disappearance of the nuclear membrane, the centrioles migrate to the opposite end and spindle formation occurs.
- Till prophase, the chromosomes have no particular orientation because the spindle has not yet formed.
- During late prophase or prometaphase, kinetochores appear on each side of the centromere.
- The sister chromatids are attached to the so-called kinetochore spindle fibers with the help of the kinetochore.
2. Metaphase:
- Chromosomes become fully condensed and distinct.
- Chromosomes move towards the equatorial plane of spindles or metaphase plates.
- Chromosomes are arranged with their arms directed towards pole and centromere towards the equator.
3. Anaphase:
- The centromere splits and the two chromatids of the duplicated chromosomes separate from each other.
- Each chromatid now becomes a daughter chromosome.
- Daughter chromosomes, each with a centromere and single chromatid appear to move toward opposite poles.
- Anaphase is the shortest phase of mitosis.
4. Telophase:
- During this phase, the spindle disappears and new nuclear envelopes develop around the daughter chromosomes.
- Each daughter nucleus contains the same number and kinds of chromosomes as the original parent cell.
- The chromosomes become diffuse chromatin and the nucleolus reappears.
- It is followed by the division of the cytoplasm

During Anaphase: The centromere splits and the two chromatids of the duplicated chromosomes separate from each other. Each chromatid now becomes a daughter chromosome. Daughter chromosomes, each with a centromere and single chromatid appear to move toward opposite poles.
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