Browse by Stream
QnA
Home
QnA
Go To Your Study Dashboard

Get Answers to all your Questions

header-bg qa
  • Home
  • NCERT
  • Can you think and answer how a reporter enzyme can be used to monitor transformation of host cells by foreign DNA in addition to a selectable marker?

9. Can you think and answer how a reporter enzyme can be used to monitor the transformation of host cells by foreign DNA in addition to a selectable marker?

Answers (1)

In recombinant DNA technology, the selection of transformed and non-transformed cells can be done using reporter genes that encode reporter enzymes. During the RDT experiment, the foreign gene is joined with a reporter gene. The reporter gene should be such that it produces a visible expression. For example, the Lac Z gene which codes for the enzyme beta-galactosidase is used as a reporter gene. The activity of this gene is not found in transformed cells as the product formed by its catalysation is not formed in transformed cells and bacterial colonies appear white. In non-transformed cells, this gene shows its activity and the catalysed product is formed, as a result of this, bacterial colonies appear blue. Thus, a reporter enzyme can be used to monitor the transformation of host cells by foreign DNA in addition to a selectable marker.

Posted by

Sonika

View full answer

Crack CUET with india's "Best Teachers"

  • HD Video Lectures
  • Unlimited Mock Tests
  • Faculty Support
cuet_ads

Similar Questions

Trending Articles/News

anam-khan

PM Modi's Pariksha Pe Charcha 2025 to be held next month, registrations open till January 14
anam-khan

CBSE Board Exams 2025: Online portal for availing exemptions by CWSN students opens
anam-khan

Board Exams: States agree to equivalence; no question paper ‘jumbling’ from next year, says PARAKH CEO

Latest Question