-
Engineering and Architecture
Exams
Colleges
Predictors
Resources
-
Computer Application and IT
Quick Links
Colleges
-
Pharmacy
Colleges
Resources
-
Hospitality and Tourism
Colleges
Resources
Diploma Colleges
-
Competition
Other Exams
Resources
-
School
Exams
Top Schools
Products & Resources
-
Study Abroad
Top Countries
Student Visas
-
Arts, Commerce & Sciences
Exams
Colleges
Upcoming Events
Resources
-
Management and Business Administration
Exams
Colleges & Courses
Predictors
-
Learn
Online Courses
Engineering Preparation
Medical Preparation
-
Online Courses and Certifications
Top Streams
Specializations
- Digital Marketing Certification Courses
- Cyber Security Certification Courses
- Artificial Intelligence Certification Courses
- Business Analytics Certification Courses
- Data Science Certification Courses
- Cloud Computing Certification Courses
- Machine Learning Certification Courses
- View All Certification Courses
Resources
-
Medicine and Allied Sciences
Colleges
Predictors
Resources
-
Law
Resources
Colleges
-
Animation and Design
Predictors & Articles
Colleges
Resources
-
Media, Mass Communication and Journalism
Exams
Colleges
Resources
-
Finance & Accounts
Top Courses & Careers
Colleges
Get Answers to all your Questions
- #Biotechnology : Principles and Processes
- #CBSE 12 Class
- #Biology Textbook for Class XII
- #NCERT
- #Biology
11. Explain briefly :
(a) PCR
Answers (1)
Polymerase Chain Reaction (PCR)- The molecular technique to amplify a gene and obtain its several copies is referred to as PCR. The process of PCR has certain requirements i.e. a thermostable enzyme called Taq polymerase ( obtained from Thermus aquaticus), primers ( short stretches of DNA ), dNTPs, a template strand etc. The process of PCR takes place in three steps.
1. Denaturation- The double-stranded DNA helix is opened up by breaking their H-bonds at high temperature.
2. Annealing- The primers are allowed to hybridise to complementary regions of DNA. This step takes place at 45-55 C temperature.
3. Extension- The primers are extended with the help of Taq polymerase enzyme and the cycle is repeated several times to obtain the desired number of copies.
Crack CUET with india's "Best Teachers"
- HD Video Lectures
- Unlimited Mock Tests
- Faculty Support
Similar Questions
- 100 g of liquid A (molar mass 140 g mol^-1) was dissolved in 1000 g of liquid B (molar mass 180 g mol^-1).The vapour pressure of pure liquid B was found to be 500 torr.
- 100 turn rectangular coil ABCD (in X-Y plane) is hung from one arm of a balance (shown in figure). A mass 500 g is added to the other arm to balance the weight of the coil.
- 100m long antenna is mounted on a 500m tall building The complex can become a transmission tower for waves with λ (a) ~ 400 m (b) ~ 25 m (c) ~ 150 m (d) ~ 2400 m
Trending Articles/News
Latest Question
- A sum of money under compound interest doubles itself in 4 years. In how many years will it become 16 times itself? Option: 1 1
- A certain loan amounts, under compound interest, compounded annually earns an interest of Rs.1980 in the second year and Rs.2178 in the third year. How much interest did it earn in the first year?<
