11. Explain briefly :

       (a) PCR

Answers (1)
S Sonika

Polymerase Chain Reaction (PCR)- The molecular technique to amplify a gene and obtain its several copies is referred to as PCR. The process of PCR has certain requirements i.e. a thermostable enzyme called Taq polymerase ( obtained from Thermus aquaticus), primers ( short stretches of DNA ), dNTPs, a template strand etc. The process of PCR takes place in three steps. 

1. Denaturation- The double-stranded DNA helix is opened up by breaking their H-bonds at high temperature. 

2. Annealing- The primers are allowed to hybridise to complementary regions of DNA. This step takes place at 45-55 C temperature.

3. Extension- The primers are extended with the help of Taq polymerase enzyme and the cycle is repeated several times to obtain the desired number of copies. 

 

Preparation Products

JEE Main Rank Booster 2021

This course will help student to be better prepared and study in the right direction for JEE Main..

₹ 13999/- ₹ 9999/-
Buy Now
Rank Booster NEET 2021

This course will help student to be better prepared and study in the right direction for NEET..

₹ 13999/- ₹ 9999/-
Buy Now
Knockout JEE Main April 2021 (Easy Installments)

An exhaustive E-learning program for the complete preparation of JEE Main..

₹ 4999/-
Buy Now
Knockout NEET May 2021

An exhaustive E-learning program for the complete preparation of NEET..

₹ 22999/- ₹ 14999/-
Buy Now
Knockout NEET May 2022

An exhaustive E-learning program for the complete preparation of NEET..

₹ 34999/- ₹ 24999/-
Buy Now
Exams
Articles
Questions