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  • (a) Explain the significance of ‘palindromic nucleotide sequence’ in  the formation of recombinant DNA.  (b) Write the use of restriction endonuclease in the above process.    <div style="float:le

(a) Explain the significance of ‘palindromic nucleotide sequence’ in  the formation of recombinant DNA. 
(b) Write the use of restriction endonuclease in the above process. 

 

 

 

 
 
 
 
 

Answers (1)

(a) The palindromic nucleotide sequence is the recognition (specific) sequence present both on the vector and on a desired / alien DNA for the action of the same (specific) restriction endonuclease to act upon.

(b) Same restriction endonuclease binds to both the vector and the foreign DNA, cut each of the two strands of the double helix at specific points in their sugar-phosphate backbone of recognition sequence for restriction endonuclease / palindromic sequence of vector and foreign DNA, to cut strand a little away from the centre of the palindrome sites, creates overhanging stretches or sticky ends.

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Priyanka Kumari

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