(a) Name the type of DNA that forms the basis of DNA fingerprinting and mention two features of this DNA.

(b) Write the steps carried out in the process of DNA fingerprinting technique, and mention its application.

 

 
 
 
 
 

Answers (1)
S safeer

a) Satellite DNA

b) Steps carried out in the process of DNA fingerprinting technique are:

  • Isolation of DNA from the sample,
  • digestion of DNA by restriction endonuclease enzyme,
  • separation of DNA fragments by electrophoresis process,
  • blotting of separated DNA fragments to synthetic membranes such as nitrocellulose or nylon,
  • hybridisation by using labelled VNTR probe,
  • And at last the detection of hybridised DNA fragments by autoradiography.

Application of DNA fingerprinting technique- In forensic labs for detection and testings.

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