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  • Explain how 'sticky ends' are obtained in a DNA segment. Write their importance in DNA technology.         <di

Explain how 'sticky ends' are obtained in a DNA segment. Write their importance in DNA technology.

 

 

 

 
 
 
 
 

Answers (1)

Restriction enzymes cut the strand of DNA a little away from the center of the palindrome sites, but between the same two bases on the opposite strands. This leaves single-stranded portions at the ends. There are overhanging stretches called sticky ends on each strand.

These are named so because they form hydrogen bonds with their complementary cut counterparts. This stickiness of the ends facilitates the action of the enzyme DNA ligase.

When foreign DNA and vector DNA is cut by the same restriction enzyme, the resultant DNA fragments have the same kind of ‘sticky-ends’ and, these can be joined together (end-to-end) using DNA ligases.
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Posted by

Priyanka Kumari

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