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  • Explain the process of gel-electrophoresis technique.           <div style="floa

Explain the process of gel-electrophoresis technique.


 

 

 

 

 
 
 
 
 

Answers (1)

The cutting of DNA by restriction endonucleases results in fragments of DNA and these fragments are separated by gel electrophoresis.

  • DNA molecules are negatively charged, they are separated by forcing them to move towards the anode under an electric field through a medium/matrix. (Agarose)
  • The DNA fragments resolve according to their size through receiving effect provided by the agarose gel. The smaller, the fragment size, the further it moves.
  • The separated DNA fragments can be visualized after staining the DNA with Ethidium bromide followed by exposure to UV.
  • Bright orange colour bands are observed.
  • The separated bands of DNA are cut from the agarose gel and extracted from the gel piece and this step is known as elution.
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Priyanka Kumari

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