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  • -galactosidase enzyme site is a preferred selectable marker in comparison to antibiotic resistant selectable marker in biotechnology experiments. Justify.  </

\beta-galactosidase enzyme site is a preferred selectable marker in comparison to antibiotic resistant selectable marker in biotechnology experiments. Justify.

 

 

 

 
 
 
 
 

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When a gene of interest is inverted within the coding sequence \beta- galactosidase enzyme if the recipient cells fail to develop the blue colour in the presence of a chromogenic substrate. It is an indication of successful recombinant formation and present of the blue colour show no recombinants formed, whereas, antibiotic-resistant selectable markers used, as it requires simultaneously planting of two different antibiotics to differentiate between transformants from non-transformants to identify the recombinants from them, which is a recombinant from them. It is a very time consuming and cumbersome porous in comparison to the first one.

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Priyanka Kumari

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