Browse by Stream
QnA
Home
QnA
Go To Your Study Dashboard

Get Answers to all your Questions

header-bg qa
  • Home
  • School
  • Restriction endonucleases have played a very significant role in rDNA technology. Explain the roles of EcoRI and DNA ligase in formation of recombinant DNA.       <di

Restriction endonucleases have played a very significant role in rDNA technology. Explain the roles of EcoRI and DNA ligase in formation of recombinant DNA.

 

 

 

 
 
 
 
 

Answers (1)

Restriction enzymes cut the strand of DNA a little away from the centre of the palindrome sites, but between the same two bases on the opposite strands. This leaves single-stranded portions at the ends. There are overhanging stretches called sticky ends on each strand. This stickiness of the ends facilitates the action of the enzyme DNA ligase.

Restriction endonucleases are used in genetic engineering to form ‘recombinant’ molecules of DNA, which are composed of DNA from different sources/genomes. When cut by the same restriction enzyme, the resultant DNA fragments have the same kind of ‘sticky-ends’ and, these can be joined together (end-to-end) using DNA ligases.
E.coR 1 Recognition sequence- 5’---GAATTC--- 3’
                                                   3’---CTTAAG---5’

Posted by

Priyanka Kumari

View full answer

Crack CUET with india's "Best Teachers"

  • HD Video Lectures
  • Unlimited Mock Tests
  • Faculty Support
cuet_ads

Similar Questions

Trending Articles/News

anam-khan

CBSE Class 10, 12 practical exams 2025 for compartment students from July 10; detailed guidelines issued
anam-khan

CBSE Central Sector Scheme of Scholarship 2025 applications open on scholarships.gov.in
anam-khan

CBSE Board 2025: Class 10th, 12th supplementary date sheet out; exams from July 15

Latest Question