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  • Study the figure of vector pBR322 given below. <img src="data:image/png;base64,iVBORw0KGgoAAAANSUhEUgAAAooAAAJSCAYAAABEGeTOAAAgAElEQVR4nO3dv8v0ynn/8fs/SWNIEZyDCwVSpDMBp5FduEivQJoUp1CaOBACKkMKQVyrOo1FapUBNUkjyKmES6VVq3a+xfOdPaPZkVba1Y+5Zt4vWLDPc/

Study the figure of vector pBR322 given below.

Identify A, B and C and explain their roles in cloning a vector.

 

 

 

 
 
 
 
 

Answers (1)

pBR322 is one of the first-ever plasmid, which was used for cloning. It has genes that codes for resistance to ampicillin and tetracycline.

Site A: amp
Role: This site codes for ampicillin resistance.

Site B: ori
Role: It is the site where DNA replication begins which enables the plasmid to reproduce itself to survive within the cell.

Site C: rop Role:
rop stands for “regulator of origin of plasmid” this site is involved in the regulation of copy of a number of pBR322.

Site D: tet Role: This site code for tetracycline resistance.

Posted by

Priyanka Kumari

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