The principle of column chromatography is:

  • Option 1)

    Gravitational force

  • Option 2)

    Capillary action

  • Option 3)

    Differential absorption of the substances on the solid phase.

  • Option 4)

    Differential adsorption of the substances on the solid phase.

Answers (2)

The main principle involved in column chromatography is adsorption of

the solutes of a solution through a stationary phase & separates the 

mixture into individual components. This is based on the affinity towards

the mobile phase & stationary phase.

\therefore option (4) is correct. 


Option 1)

Gravitational force

Option 2)

Capillary action

Option 3)

Differential absorption of the substances on the solid phase.

Option 4)

Differential adsorption of the substances on the solid phase.

Column chromatography is a types of chromatography commonly used for the isolation or purification of the solutes.  Column chromatography has two phases in which the mobile phase is liquid and solid stationary phase is used, mobile phase and stationary phase. Solute adsorption through a solid stationary phase isolates the mixture into its component, which relies on the affinity between the stationary phase and the mobile phase. Molecules that have low affinity toward the silica they eluted rapidly while the molecules that have a higher affinity are strongly retained.

Reference:Principle and Procedure of Column Chromatography

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