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The total number of negative charge in the tetrapeptide, Gly-Glu-Asp-Tyr, at pH 12.5 will be______. (Integer answer)
 

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The amino acids are

Gly : Glycine :

\text{NH}_{2}-\text{CH}_{2}-\text{COOH}

Glu : Glutamic acid :

Asp : Aspartic acid:


Tyr : Tyrosine:

Therefore, the given tetrapeptide is

Therefore, at pH = 12.5,  the carboxylic acids as well as the phenolic group will be deprotonated.

Thus ,the tetrapeptide will carry (-4) charge

Hence, the answer is 4

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sudhir.kumar

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