The total number of negative charge in the tetrapeptide, Gly-Glu-Asp-Tyr, at pH 12.5 will be______. (Integer answer)
The amino acids are
Gly : Glycine :
Glu : Glutamic acid :
Asp : Aspartic acid:
Tyr : Tyrosine:
Therefore, the given tetrapeptide is
Therefore, at pH = 12.5, the carboxylic acids as well as the phenolic group will be deprotonated.
Thus ,the tetrapeptide will carry (-4) charge
Hence, the answer is 4
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