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- Which of the following is the main principle behind the Biuret test?Option: 1 Formation of a peptide bond between two adjacent amino acids in a prote
Which of the following is the main principle behind the Biuret test?
Formation of a peptide bond between two adjacent amino acids in a protein.
Formation of a complex between the Biuret reagent and the amino group of an amino acid.
Formation of a coloured complex between the Biuret reagent and reducing sugar.
Formation of a precipitate in the presence of a specific protein.
Answers (1)
The Biuret test is a biochemical assay used to detect the presence of proteins in a sample. The test is based on the principle that when the Biuret reagent, which is a solution of copper sulfate and sodium hydroxide, is added to a solution containing proteins, it reacts with the peptide bonds between adjacent amino acids in the protein to form a colored complex. This complex is the basis for the detection of proteins using the Biuret test. The intensity of the color produced is proportional to the concentration of proteins in the sample.
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