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A group of scientists is working on a project that uses recombinant DNA technology. For their experiment, they must choose a restriction enzyme. They should use a type II restriction endonuclease enzyme. They've narrowed it down to two enzymes: EcoRI and Sau3AI. Which enzyme, would be the best fit for their experiment?
EcoRI
Sau3AI
EcoRI and Sau3AI are both viable options.
Neither EcoRI nor Sau3AI are restriction endonucleases of type II.
Answers (1)
The scientists should use a Type II restriction endonuclease enzyme when selecting a restriction enzyme for their experiment. EcoRI and Sau3AI are both restriction enzymes of type II.
EcoRI is a bacterium-derived enzyme that recognises the palindromic DNA sequence 5'-GAATTC-3'. It cuts the DNA between the G and A in that sequence.
Sau3AI, a Type II restriction endonuclease, recognises a distinct DNA sequence. Sau3AI's recognition sequence is not palindromic and differs from EcoRI's.
Since both EcoRI and Sau3AI are Type II restriction enzymes, they are both viable options for the scientists' experiment. The choice between the two would depend on the specific requirements of their experiment, such as the desired DNA sequence to be cleaved or other experimental considerations.Hence, option 3 is the correct answer.
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