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  • Answer the following questions based on Meselson and Stahl’s experiment on E. coli : (a) Write the name of the chemical substance used as the only source of nitrogen in the experiment. (b) Why did they allow the synthesis

Answer the following questions based on Meselson and Stahl’s experiment on E. coli :


(a) Write the name of the chemical substance used as the only source of nitrogen in the experiment.
(b) Why did they allow the synthesis of the light and the heavy DNA molecules in the organism?
(c) How did they distinguish the heavy DNA molecules from the light DNA molecules? Explain.
(d) Write the conclusion the scientists arrived at, at the end of the experiment.

 

 

 

 
 
 
 
 

Answers (1)

(a) Ammonium chloride (NH4Cl).

(b) To check if DNA replication was semi-conservative.

(c) The heavy and light DNA molecules were distinguished by centrifugation in a cesium chloride density gradient. 

(d) The scientists concluded that DNA replicates semi-conservatively.

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Satyajeet Kumar

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