Assertion (A): The vector should have relatively few, ideally single, recognition sites for the commonly employed restriction enzymes in order to link the foreign DNA).
Reason (R): The presence of multiple recognition sites inside the vector will result in various fragments, complicating gene cloning.
Both A and R are true and R is the correct explanation of A.
Both A and R are true but R is not the correct explanation of A.
A is true but R is false.
A is false but R is true.
Both A and R are false.
Exonucleases and endonucleases are the two types of restriction enzymes. Exonucleases remove nucleotides from the j ends of DNA, whereas endonucleases cut the DNA at particular locations.
The presence of multiple recognition sites inside the vector will result in various fragments, complicating gene cloning. As a result, the vector must have relatively few, ideally single, recognition/cloning sites for the commonly employed restriction enzymes in order to link the foreign DNA.
Option a is the correct answer.