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Assertion (A): The vector should have relatively few, ideally single, recognition sites for the commonly employed restriction enzymes in order to link the foreign DNA).

Reason (R): The presence of multiple recognition sites inside the vector will result in various fragments, complicating gene cloning.

 

Option: 1

Both A and R are true and R is the correct explanation of A.
 


Option: 2

Both A and R are true but R is not the correct explanation of A.


Option: 3

A is true but R is false.


Option: 4

A is false but R is true.

 


Option: 5

Both A and R are false.


Answers (1)

best_answer

Exonucleases and endonucleases are the two types of restriction enzymes. Exonucleases remove nucleotides from the j ends of DNA, whereas endonucleases cut the DNA at particular locations. 

The presence of multiple recognition sites inside the vector will result in various fragments, complicating gene cloning. As a result, the vector must have relatively few, ideally single, recognition/cloning sites for the commonly employed restriction enzymes in order to link the foreign DNA.

Option a is the correct answer. 

 

Posted by

Suraj Bhandari

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