Considering the cloning process involving gene ‘G’ and vector ‘V’, the student employed specific restriction enzymes (RE).
Following the gene and vector cuts with RE1 and RE3. In further addition, the plasmid L is treated with RE2 and exhibited a band of approximately 6.6 kb.
The following three propositions were put forth to account for this finding that is,
1. Plasmid L is essentially indistinguishable from vector V since solely the uncut V was introduced into E. coli.
2. Gene G has the RE2 recognition site.
3. The restriction enzymes RE1 and RE2 demonstrate iso-schizomeric properties.
Which of the following combinations is correct?
1 only, neither 2 nor 3
2 only, neither 1 nor 3
Either 2 or 3, but not 1
3 only, neither 1 nor 2