During DNA replication, Which of the following reactions is required for proofreading by DNA Pol III?
5’ to 3’ exonuclease activity
3’ to 5’ exonuclease activity
5’ to 3’ endonuclease activity
3’ to 5’ endonuclease activity
As we have already discussed in DNA Replication in Prokaryotes -
DNA Polymerase III shows 3’-5’ exonuclease proofreading activity. It helps in the removal of mispaired nucleotides so that the polymerase begins again. Hence, the correct option is (b).