Following statements describe the characteristics of the enzyme Restriction Endonuclease. Identify the incorrect statement.

  • Option 1)

    The enzyme cuts DNA molecule at identified position within the DNA.

  • Option 2)

    The enzyme binds DNA at specific sites and cuts only one of the two strands.

  • Option 3)

    The enzyme cuts the sugar-phosphate backbone at specific sites on each strand.

  • Option 4)

    The enzyme recognises a specific palindronic nucleotide sequence in the DNA.

Answers (1)

 

Restriction Endonuclease -

These enzymes cleave DNA only within or near the specific base sequence. These sequences are called as recognition sites. They are of three types RE- I, RE-II and RE-III.

- wherein

1st Restriction enzyme (Hind II) was used in RDT. 

 

 

Restriction endonuclease bind to the specific sites (palindromic sequences) the DNA and cut both the strands of the DNA.


Option 1)

The enzyme cuts DNA molecule at identified position within the DNA.

Option 2)

The enzyme binds DNA at specific sites and cuts only one of the two strands.

Option 3)

The enzyme cuts the sugar-phosphate backbone at specific sites on each strand.

Option 4)

The enzyme recognises a specific palindronic nucleotide sequence in the DNA.

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