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Get Answers to all your Questions
The correct order of Polymerase Chain Reaction (PCR) is
- Option 1)
Denaturation , Extension , Annealing
- Option 2)
Annealing , Extension , Denaturation
- Option 3)
Extension , Denaturation , Annealing
- Option 4)
Denaturation , Annealing , Extension
Answers (2)
As we learnt in
PCR -
The polymerase chain Reaction (PCR) was invented by Kary Mullis (1985). In PCR, Template DNA, Oligonucleotides or primers, Taq polymerase and dNTP, are used to amplify DNA
- wherein
PCR is used to amplify (many copies) targeted sequence
Polymerase Chain Reaction (PCR), technique is used for making multiple copies of gene (or DNA) of interest in vitro. Each cycle has three steps
(i) Denaturation
(ii) Primer annealing
(iii)Extension of primer
Option 1)
Denaturation , Extension , Annealing
This is incorrect.
Option 2)
Annealing , Extension , Denaturation
This is incorrect.
Option 3)
Extension , Denaturation , Annealing
This is incorrect.
Option 4)
Denaturation , Annealing , Extension
This is correct.
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