Given figure represents the steps involved in polymerase chain reaction (PCR). Identify the steps A ,B ,C and D and select the correct option
A - Denaturation at 94-96oC
B - Extension through Taq polymerase at 72oC
C - Repetition of denaturation and annealing
D - Annealing at 72oC
A - Denaturation at 94-96oC
B - Annealing at 40-60oC
C - Extension through Taq polymerase at 72oC
D - Repetition of denaturation and annealing
A - Denaturation at 40-60oC
B -
C - Extension through Taq polymerase at 94-96oC
D - Repetition of denaturation and annealing
A - Extension through Taq polymerase at 72oC
B - Denaturation at 40oC
C - Annealing at 72oC
D - Repetition of denaturation and annealing
As we have learned
Steps in PCR -
PCR is performed in a temperature controlling machine, called as thermocycler. Denaturation, primer annealing and primer extension are three steps of PCR
Hence, the correct option is (b).