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Given figure represents the steps involved in polymerase chain reaction (PCR). Identify the steps A ,B ,C and D and select the correct option 

Option: 1

A - Denaturation at 94-96oC

B - Extension through Taq polymerase at 72oC

C - Repetition of denaturation and annealing

D - Annealing at 72oC


Option: 2

A - Denaturation at 94-96oC

B - Annealing at 40-60oC

C - Extension through Taq polymerase at 72oC

D - Repetition of denaturation and annealing


Option: 3

A - Denaturation at 40-60oC

B -

C - Extension through Taq polymerase at 94-96oC

D - Repetition of denaturation and annealing


Option: 4

A - Extension through Taq polymerase at 72oC

B - Denaturation at 40oC

C - Annealing at 72oC

D - Repetition of denaturation and annealing


Answers (1)

best_answer

As we have learned

Steps in PCR -

PCR is performed in a temperature controlling machine, called as thermocycler. Denaturation, primer annealing and primer extension are three steps of PCR

Hence, the correct option is (b).

 

 

 

Posted by

sudhir.kumar

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